Airborne rhinovirus detection and effect of ultraviolet irradiation on detection by a semi-nested RT-PCR assay
نویسندگان
چکیده
منابع مشابه
Airborne rhinovirus detection and effect of ultraviolet irradiation on detection by a semi-nested RT-PCR assay
BACKGROUND Rhinovirus, the most common cause of upper respiratory tract infections, has been implicated in asthma exacerbations and possibly asthma deaths. Although the method of transmission of rhinoviruses is disputed, several studies have demonstrated that aerosol transmission is a likely method of transmission among adults. As a first step in studies of possible airborne rhinovirus transmis...
متن کاملDetection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...
متن کاملNested-RT-PCR and multiplex RT-PCR for diagnosis of rhinovirus infection in clinical samples.
Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods hav...
متن کاملFirst Molecular Detection and Vp7 (g) Genotyping of Group a Rotavirus by Semi-nested Rt-pcr from Sewage in Nigeria
Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 6000 and dextran was used to concentrate sewage ...
متن کاملRT-PCR Detection of Coxsackievirus B3: A Viral Myocarditis
Backgrounds and Aims: Coxsakievirus B3 (CVB3), one of the six Coxsakievirus B serotypes, is a member of the Enterovirus genus within the Picornaviridae family. CVB3 is an important pathogen of viral myocarditis, which accounts for more than 50% of viral myocarditis cases. The genome of CVB3, like that of other Entroviruses, is a single-stranded, sense, polyadenylated RNA molecule with 7400 nucl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Public Health
سال: 2003
ISSN: 1471-2458
DOI: 10.1186/1471-2458-3-5